腺样囊性癌 TrkC 差异表达的 PDX 模型的建立与 鉴定

目的：建立并鉴定腺样囊性癌（adenoid cystic carcinoma，ACC）关于原肌球蛋白受体激酶 C（trypomyosin-receptor kinase C，TrkC）差异表达的人源肿瘤异种移植模型（patient-derived tumor xenograft，PDX）。方法：收集北京同仁 医院头颈外科收治的腺样囊性癌患者的新鲜肿瘤组织，皮下接种至免疫缺陷鼠体内，并不断传代至新一批的小鼠体 内直至肿瘤稳定生长。分析患者 ACC 肿瘤组织在免疫缺陷动物体内自然生长状态下的组织病理特征。结果：本次 实验共收集 ACC 标本 10 例，其中 5 例成功建立 PDX 模型，3 例完成鉴定。建立的 PDX 模型病理形态和组织结构与 患者肿瘤组织保持一致；3 例 PDX 模型 TrkC 表达呈现不同的特点。结论：PDX 模型可以很好地保留患者的肿瘤异 质性，有助于深入研究 ACC 发病机理，为以 TrkC 为靶点的临床前药物筛选提供动物模型。

［关键词］腺样囊性癌；人源肿瘤异种移植模型；生长速度；TrkC

［中图分类号］R739.87；R73-35 +2 ［文献标志码］A doi：10.3969 ／j.issn.1674-0904.2020.03.002

引文格式：Ma TY，Wang XL，Chen XH，et al.Establishment and identification of PDX model for differentially expressed of TrkC in adenoid cystic carcinoma［J］.J Cancer Control Treat，2020，33（3）:208 -214.［马廷耀，王雪莲，陈晓红，等.腺样囊性癌 TrkC 差异表达的 PDX 模型的建立与鉴定［J］.肿瘤预防与治疗，2020，33（3）:208 -214.］

Establishment and Identification of PDX Model for Differentially EXpressed of TrkC in Adenoid Cystic Carcinoma

Ma Tingyao# ，Wang Xuelian# ，Chen Xiaohong，Xu Chunping，Wu Jun，Jiang Lingyan，Xu Hongbo，Zhou Jing，Song Xiaoming，Li Wenwei # Contributed equally Department ofOtolaryngology，Beijing Tongren Hospital，Capital Medical University，Beijing 100730，China （Ma Tingyao，Wang Xuelian，Chen Xiaohong，Wu Jun，Jiang Lingyan，Xu Hongbo，Zhou Jing）；Yicon （Beijing）Medical Science And Technology Co.，Ltd.，Beijing 100716，China （Xu Chunping，Song Xiaoming，Li Wenwei）

Corresponding author：Chen Xiaohong，E-mail：trchxh@163.com This study was supported by grants from Beijing Municipal Administration of Hospital （NO. XMLX201701）
［Abstract］ Objective：To establish and identify a patient-derived tumor xenograft （PDX）model for the differential expression of TrkC in adenoid cystic carcinoma （ACC）.Methods：Fresh tumor tissue of patients with ACC treated in the department of otolaryngology in Beijing Tongren Hospital were collected，subcutaneously inoculated into immunodeficient mice， and continuously passed into a new batch of mice until the tumors stably grew.The histopathological characteristics of ACC tissue in natural state of immunodeficient animals were analyzed.Results：Ten ACC samples were collected in this experiment.PDX models were successfully established for 5 cases.Pathology，morphology and structure of established PDX models were consistent with those of patients’tumor tissue；TrkC expression in the 5 PDX models showed differ characteristics.Conclusion：The PDX model can well preserve the tumor heterogeneity，which is conducive to the in-depth study of the pathogenesis of ACC，and provides an animal model for preclinical screening of Trkc-targeted drugs. ［Key Words］ Adenoid cystic carcinoma；Patient-derived tumor xenograft models；Growth rate；NT-3；TrkC